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Antiviral methods and protocols / edited by Derek Kinchington and Raymond F. Schinazi.

Contributor(s): Kinchington, Derek | Schinazi, Raymond F.
Material type: TextTextSeries: Methods in molecular medicine: 24.Publisher: Totowa, N.J. : Humana Press, c2000General Notes: Available through the EBSCO e-book Collection, which can be found on the Davenport University Library database page.Bibliography: Includes bibliographical references and index.Description: 1 online resource (xiv, 404 p.) : ill.Content type: text Media type: comptuer Carrier type: online resourceISBN: 9780585235684 ; 9781592592456 .Subject(s): Antiviral agents -- Laboratory manuals | Hepatitis, Viral -- Drug therapy | Antiviral agents -- Therapeutic use | DNA viruses -- Drug therapy | RNA viruses -- Drug therapyGenre/Form: Electronic books DDC classification: 616.9/25061 Online resources: Access full-text materials at no charge. Also available in print.
Contents:
Changing methods for discovering antiviral drugs -- Laboratory safety considerations -- Routine quality control testing of cell cultures : Detection of mycoplasma -- Hep ad38 assay : A high -- throughput, cell-based screen for the evaluation of compounds against hepatitis b virus -- Hepatitis b virus cell culture assays for antiviral activity -- A rapid microtiter assay for duck hepatitis virus reverse transcriptase -- Assays for measuring hepadnaviral supercoiled DNA -- Assays for hepatitis b virus DNA-and-RNA-dependent DNA polymerase activities -- Development of a hepatitis c virus RNA helicase high throughput assay -- Determination of viral infectivity : HSV, VZV, and CMV -- Antiviral screening assays for human herpesviruses 6 and 7 -- Strategies for evaluation of antiviral agents against Epstein-Barr virus in culture -- Monitoring drug resistance for herpesviruses -- Herpesvirus protease assays -- Testing compounds for antiviral activity in cell cultures infected with HIV -- Evaluation of compounds that prevent reactivation of hiv-1 in om-10.1 cells -- Assessment of activity of topical virucidal agents -- Assays that measure selective killing of virus-infected cells -- Evaluating clinical isolates for their phenotypic and genotypic resistance against anti-HIV drugs -- Line probe assay for detecting mutations in hiv-1 reverse transcriptase – Rt-PCR for amplification of specific fragments of hiv-1 genome -- Evaluation of compounds against recombinant HIV reverse transcriptase -- Ultrasensitive detection of reverse transcriptase activity by the amp-rt assay : Applications to the measurement of virus loads and phenotypic drug resistance -- Reverse transcriptase assay based on product enhancement for assessing the drug susceptibility of retroviruses -- Identifying and characterizing HIV protease inhibitors -- Retroviral integrase : A novel target in antiviral drug development and basic in vitro assays with the purified enzyme -- Papillomavirus DNA replication -- Quantitative immunodetection of viral oncoprotein levels in human papillomavirus -- transformed human cells -- Biochemical methods for the characterization of influenza viruses with reduced sensitivity to 4-guanidino-neu5ac2en -- Virological methods for the generation and characterization of influenza viruses with reduced sensitivity to 4-guanidino-neu5ac2en.
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Available through the EBSCO e-book Collection, which can be found on the Davenport University Library database page.

Includes bibliographical references and index.

Changing methods for discovering antiviral drugs -- Laboratory safety considerations -- Routine quality control testing of cell cultures : Detection of mycoplasma -- Hep ad38 assay : A high -- throughput, cell-based screen for the evaluation of compounds against hepatitis b virus -- Hepatitis b virus cell culture assays for antiviral activity -- A rapid microtiter assay for duck hepatitis virus reverse transcriptase -- Assays for measuring hepadnaviral supercoiled DNA -- Assays for hepatitis b virus DNA-and-RNA-dependent DNA polymerase activities -- Development of a hepatitis c virus RNA helicase high throughput assay -- Determination of viral infectivity : HSV, VZV, and CMV -- Antiviral screening assays for human herpesviruses 6 and 7 -- Strategies for evaluation of antiviral agents against Epstein-Barr virus in culture -- Monitoring drug resistance for herpesviruses -- Herpesvirus protease assays -- Testing compounds for antiviral activity in cell cultures infected with HIV -- Evaluation of compounds that prevent reactivation of hiv-1 in om-10.1 cells -- Assessment of activity of topical virucidal agents -- Assays that measure selective killing of virus-infected cells -- Evaluating clinical isolates for their phenotypic and genotypic resistance against anti-HIV drugs -- Line probe assay for detecting mutations in hiv-1 reverse transcriptase – Rt-PCR for amplification of specific fragments of hiv-1 genome -- Evaluation of compounds against recombinant HIV reverse transcriptase -- Ultrasensitive detection of reverse transcriptase activity by the amp-rt assay : Applications to the measurement of virus loads and phenotypic drug resistance -- Reverse transcriptase assay based on product enhancement for assessing the drug susceptibility of retroviruses -- Identifying and characterizing HIV protease inhibitors -- Retroviral integrase : A novel target in antiviral drug development and basic in vitro assays with the purified enzyme -- Papillomavirus DNA replication -- Quantitative immunodetection of viral oncoprotein levels in human papillomavirus -- transformed human cells -- Biochemical methods for the characterization of influenza viruses with reduced sensitivity to 4-guanidino-neu5ac2en -- Virological methods for the generation and characterization of influenza viruses with reduced sensitivity to 4-guanidino-neu5ac2en.

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